7/17/2018

Raw centroids

  • median value of markers in each cluster

Normalized centroids

  • median z-score of markers in each cluster

Freq cluster

  • median z-score of markers in each cluster

Freq population

  • median z-score of markers in each cluster

Meta Phenograph +Meta tsne

  • colored by “meta” phenograph cluster

Meta Phenograph +Meta tsne

  • colored by percent of cells that are naive in original clusters
  • keep slide 4 in mind

Meta Phenograph +Meta tsne

  • colored by percent of cells that are effector in original clusters

Meta Phenograph +Meta tsne

  • colored by percent of cells that are effector memory in original clusters

Meta Phenograph +Meta tsne

  • colored by percent of cells that are central memory in original clusters

Meta Phenograph +Meta tsne

  • colored by median CD45 (scaled) expression in original clusters

Meta Phenograph +Meta tsne

  • colored by median CCR7 (scaled) expression in original clusters

Meta Phenograph +Meta tsne

  • colored by median CD28 (scaled) expression in original clusters

Meta Phenograph +Meta tsne

  • colored by median CD95 (scaled) expression in original clusters